37+ Neb Ligation Calculator

Web Ligation Calculator Determines the optimal insert amount for a cloning reaction based on vector and insert properties. Web Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.

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Set up the following reaction in a microcentrifuge tube on ice.

. The T4 DNA Ligase Buffer should be thawed and resuspended at room temperature. Web Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Web 375 ng 0060 pmol Nuclease-free water.

Note that the table shows a ligation using a molar ratio of 13. Select the product group of the polymerase or kit you plan to use. Convert double strand DNA ds DNA to pmoles and ends.

Web The NEBioCalculator Ligation module can help you plan a successful ligation reaction as part of a restriction cloning workflow by optimizing the molar ratio between your vector. Select thermostable ligase and reaction. Refer to the text below the tool for more.

T4 DNA Ligase should be added last. Web Ligation Calculator是吾爱网友基于NEB在线计算工具而开发的分子克隆之DNA连接片段用量计算器使用时需要按次序输入载体用量ng可以是整型数值或. Are you carrying out the ligation reaction under high salt conditions 100 mM NaCl.

Ds DNA size in base pairs bp Quantity in micogram ug PART 2. Web The NEBioCalculator Ligation module can help you plan a successful ligation reaction as part of a restriction cloning workflow by optimizing the molar ratio between your vector. Web Use this tool to estimate an appropriate incubation temperature when using NEB thermostable ligases and reaction buffers.

For example in NEBuffer 31. Web The NEBioCalculator Ligation module can help you plan a successful ligation reaction as part of a restriction cloning workflow by optimizing the molar ratio between your vector. Select the product group of the polymerase or kit you plan to use.

Web If your insert is smaller than the vector say if youre trying to ligate a 1kb insert into a 3kb vector youll need a higher ratio in this case about a 31 molar ratio of insert to vector. Single base 1 nt TA overhangs. Select thermostable ligase and reaction.

Web Use this tool to estimate an appropriate incubation temperature when using NEB thermostable ligases and reaction buffers.

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